This pilot study demonstrates that Raman fluorescence diagnostics (RFD) is a feasible, objective, and non-invasive tool for assessing dental hard tissue mineralization and oral hygiene status in patients with orthodontic braces. In a single-center, single-arm pilot trial (n = 40), a statistically significant reduction in enamel mineralization was observed during brace wear, with recovery to baseline levels after remineralization therapy. RFD showed high sensitivity (92-95%) and specificity (90-94%) and enabled personalized hygiene monitoring and patient feedback. Given the pilot nature of the study, lack of a comparator group, and single-center design, the results are preliminary and require confirmation in larger controlled trials.